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KMID : 0043319910140030217
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Archives of Pharmacal Research
1991 Volume.14 No. 3 p.217 ~ p.224
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Immunomodulating Activities of Water Extract from Xanthium strumarium (II) : Immunostimulating effects of the water layer after treated with chloroform
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Moon EY
Park SY/Ahn MJ/Ahn JW/Zee OP/Park EK
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Abstract
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One of water and/or methanol extracts from 14 herbal drugs which were screened using murine splenocytes showed immunosuppressive activities previously. After water extract from Xanthium strumarium was treated with chloroform. 100mcg/ml of water layer (XS-WC1) has very strong immunostimulating activities tested by 3H-thymidine incorporation (control vs 100mcg/ml, 4962 cpm vs 69515 cpm). MLR also appears to be stimulated strongly (control vs 100mcg/ml, 26345cpm vs 78688cpm). When 100mcg/ml of XS-WC1 and 0.8mcg/ml of concanavalin A (ConA) were added, more 3H-thymidine were incorporated significantly. compared with 0.8mcg/ml of ConA only. In contrast with ConA. results from 5mcg/ml of lipopolysaccharide (LPS) and 100mcg/ml of XS-WC1 were not different, compared with 5mcg/ml of LPS only. These results indicated that responses of XS-WC1 to B cell and T cell may be different. XS-WC1 was injected intraperitoneally (10mg/kg, 50mg/kg, l00mg/kg) for 4 days or 10 days and tested secretion of IgM or IgG by direct and indirect heinolytic plaque-forming cell assays, respectively. Numbers of hemolytic plaques for both IgM and IgG were increased significantly. Especially, secretion of IgGs was increased more than 10 times. After administration of XS-WC1 for 7 days (50mg/kg, 100mg/kg splenomegaly due to graft vs host reaction was observed. Human lymphocytes separated from whole blood by Ficoll-Hypaque method were also proliferated after treatment of 10mcg/ml and 50mcg/ml of XS-WC1. As seen in murine lymphocytes. hui-nan lymphocyte proliferation was increased synergistically after treatment with both of XS-WC1 and phytohemagglutinin (PHA). It appears that XS-WC1 may have potential immunostimulating activities and that it remains to be purified further for isolation of active components.
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KEYWORD
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